Biomedical CORE B: microscopy and mistopathology
- Histology(paraffin and frozen sections)
- Electron and Confocal Microscopy
- Photodocumentation and Widefield Microscopy
- Advanced Imaging Technologies(Lightsheet,STED,SIM,etc..)
- Image Analysis
- Immunohistochemistry and Immunocytochemistry
- Histopathological Analysis(human and mouse)
- Consultation and Training
Biomedical CORE C: epithelial cell and mucosal immunology
- Polarized epithelial cell culture
- Genetic transformations of epithelial and other cells
- Intestinal organoid conditioned media
- Flow cytometry analysis of cultured cells or tissue/organoid-derived cells
- Transepithelial electrophysiology of live cells, cell monolayers, and tissues
- Gastrointestinal organoid derivation and culture
Biomedical CORE D: gnotobiotic mice, microbiology and metagenomics
- Nucleic acid extraction/amplification
- Microbiome and microbial genome sequencing
- Microbiology
- Gnotobiotic mice, hosing, defined and complex associations
- Computation and data analysis
- Metabolic cages and monitoring, under conventional and GF states
Clinical translational research program
- Design of clinical and preclinical studies
- Statistical analysis
- Data interpretation and presentation
Bio-repository and data registry for human material
- Fresh and stored human gastrointestinal tissues, stool, and blood
- Sample-associated clinical metadata
Who can use the HDDC cores and services?
Please remember to acknowledge the Harvard Digestive Disease Center and NIH grant P30DK034854 in all publications and presentations emerging from HDDC core services, resources, technology, and expertise.