Core Scientist: Bin Bao, PhD
The Flow Cytometry Core offers advanced spectral flow cytometry and high-parameter cell analysis to support cutting-edge research in immunology, inflammation, and epithelial biology.
At the heart of the Core is the BD Symphony A5 SE, a premier spectral flow cytometer that delivers unmatched sensitivity, flexibility, and resolution for complex multiparameter experiments. This system enables deep immune profiling, spectral unmixing, and discovery-driven analysis that go far beyond conventional flow cytometry.
The BD LSR Fortessa serves as our classic, rock-solid cytometer, providing reliable and reproducible results for routine immune profiling and functional assays. Together, these instruments offer both high-dimensional innovation and dependable performance for all research needs. The Core supports a broad range of applications, including: 1) Immunophenotyping and rare cell analysis; 2) Intracellular cytokine and signaling assays; 3) Cell cycle, viability, and apoptosis studies; 4) Calcium flux and receptor internalization assays.
A BD Melody Cell Sorter is also available for gentle, high-purity sorting of specific cell populations for downstream applications (scRNAseq, CRISPR Screening, etc.).
Core scientists provide expert consultation on panel design, experimental planning, and data interpretation.
Instruments
- BD Symphony A5 SE (Spectral Flow Cytometer) – flagship high-parameter analyzer for deep immunophenotyping
- BD LSR Fortessa – classic 16-color cytometer delivering reliable and stable performance
- BD FACS Canto II – 8-color analyzer for routine applications
- BD Melody – benchtop cell sorter for targeted isolations
Fees
| Instrument | HDDC Member | BCH Non-Member | Non-BCH Non-Member | Industry |
| BD Symphony | $75/hr | $85/hr | $180/hr | $200/hr |
| BD Fortessa | $50/hr | $55/hr | $100/hr | $150/hr |
| BD Canto | $20/hr | $45/hr | $45/hr | $90/hr |
| BD Melody | $80/hr | $90/hr | $177/hr | $255/hr |
Consultation & Training
Access to the Symphony A5 SE, Fortessa, Canto and Melody require training with Bin Bao, PhD. Training will provide the new users with hands-on in instrument operation, spectral setup, and troubleshooting. After demonstrating proficiency, users may reserve and operate instruments independently, with ongoing support from Core scientist as needed.