Core Scientists: David Breault, MD, PhD; Daniel Zeve, MD, PhD
Core Technicians: Bia Frintu, BSc, MS; Elisa Saint-Denis, BSc
Core Lab Manager: Hahyung Y. Kim, BSc
Services
This service provides in-depth protocols, hands-on training, biologics, and ongoing support for the derivation and culture of gastrointestinal organoids/enteroids/mini-guts from stomach, small intestine, or colon of mouse or human.
The L-WRN cell line (L cells expressing Wnt3a, R-spondin3, and Noggin) is maintained and aliquots of conditioned media are available.
The R-Spondin cell line (293T cells expressing mouse R-Spondin1) is maintained and aliquotes of conditioned media are available.
Fees
| Organoid Services | HDDC Member | Non-Member |
| Hands-on training | No charge | $100/hr |
| Consultation | No charge | $100/hr |
| Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix – Phenol Red-Free, LDEV-Free, 10mL | $495 (15 vials per year limit, additional vials will be charged at non-member rate) | $595 |
| L-WRN Conditioned Media, 500mL | $595 (5 bottles per year limit, additional bottles will be charged at non-member rate) | $695 |
| L-WRN Conditioned Media, Antibiotic Free, 500mL | $795 | $795 |
| R-Spondin Conditioned Media, 250mL | $495 | $595 |
| Recombinant Murine Noggin (100 micrograms) | $700 | $800 |
| Invitrogen EVOS FL Auto 2 Imaging Service | No charge | $25/hr |
*Smaller volumes of conditioned media are available at a prorated rate.
Logistics
Investigators are trained in organoid culture by Core scientists and staff. The Core helps investigators to establish this technology within their own labs, by providing the resources for a “pilot experiment” using primary tissue provided by the investigator or the Core as needed. Methods include isolation of crypts/glands from primary tissues, suspending them in Matrigel, culture in defined or conditioned media, and monitoring organoid health. Methods to perform viral transduction, organoid freeze down, fluorescent imaging of live cultures and post-fixation histological applications are also available. Hands-on training, consultation, and imaging is provided to HDDC members at no charge.
Quality Control
L-WRN conditioned media used for organoid generation are routinely assayed for Wnt-3a activity.